This test measures antinuclear antibodies using Indirect Immunofluorescence Assay (IFA) on HEp-2 cells and dilutes the blood sample step-by-step to measure the precise concentration (titer) and staining pattern of autoantibodies.

Why is This Test Ordered?

1. Determine Autoantibody Levels: Measures the strength/titer of ANA in the blood to help differentiate clinically significant autoimmune disease from borderline results.
2. Diagnose Autoimmune Conditions: Used to evaluate suspected Systemic Lupus Erythematosus (SLE), Scleroderma, Mixed Connective Tissue Disease, or Sjogren’s Syndrome.
3. Monitor Disease Activity: Helps physicians assess autoantibody levels over time or confirm initial positive ANA screenings.

Preparation & Fasting

1. Fasting: Not required.
2. Medications: Inform your doctor about all prescription drugs, as certain medications can cause drug-induced positive ANA results.

Sample Collection

1. A standard blood sample drawn from a vein in your arm.

Turnaround Time

1. Results are typically available within 24 to 48 hours.